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mitotracker red cmxros  (Thermo Fisher)


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    Structured Review

    Thermo Fisher mitotracker red cmxros
    Mitotracker Red Cmxros, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mitotracker+red+cmxros/Triton+X-100/pm42134333-199-29-32
    Average 99 stars, based on 1 article reviews
    mitotracker red cmxros - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: CircNCX1 contributes to mitochondrial fusion in cardiomyocyte by mediating the expression of mitofusin 2.
    Article Snippet: Mature mammalian cardiomyocytes highly utilize fatty acid-dependent aerobic respiration to produce energy effectively.. However, even embryonic and neonatal cardiomyocytes prefer anaerobic glycolysis.. The underlying molecular mechanism of this transformation during cardiomyocyte maturation hasn’t been fully understood.

    Article Title: MICU2 controls mitochondrial calcium signaling and migration in neurons during development
    Article Snippet: .. DIV11–15 neurons on 18 or 25-mm coverslips were incubated for 10 min at RT with 200 nM MitoTracker Red CMXROS (Thermo Fisher Scientific, M46752) followed by 3 × 5 min washes with Hanks’ balanced salt solution (HBSS; Gibco, 14025076) and fixation in 4% PFA (Electron Microscopy Sciences, 15714S) prewarmed to 37°C for 15 min at RT. .. Further, the cells were washed 3 × 5 min with PBS, blocked for 1 h at RT in PBS containing 3% BSA, 5% chicken serum, and 0.2% Triton x-100, and stained with primary antibodies (anti-MICU2, Bethyl, A300-BL19212, 1:200) for 45 min at RT.

    Article Title: Modeling the cell biology of PEX11β deficiency during human neurogenesis
    Article Snippet: Cells were washed once with 1X PBS and sealed with Fluoromount-G slide mounting medium (Thermo Fisher Scientific #00-4958-02). .. For MitoTracker experiments, cells were incubated with MitoTracker Red CMXRos (Thermo Fisher Scientific #M46752) at a concentration of 100nm for 30 minutes at 37°C and 5% CO2 prior to fixation. .. Neural rosettes were fixed on day 8 of differentiation with 100% ice-cold methanol (Thermo Fisher Scientific #A454-4) for 10 minutes at -20°C.

    Staining:

    Article Title: CircNCX1 contributes to mitochondrial fusion in cardiomyocyte by mediating the expression of mitofusin 2.
    Article Snippet: Mature mammalian cardiomyocytes highly utilize fatty acid-dependent aerobic respiration to produce energy effectively.. However, even embryonic and neonatal cardiomyocytes prefer anaerobic glycolysis.. The underlying molecular mechanism of this transformation during cardiomyocyte maturation hasn’t been fully understood.

    Article Title: Transplantation of Mitochondria isolated from iPSC-MSCs mitigates doxorubicin-induced cardiomyopathy by inhibiting cardiomyocyte senescence.
    Article Snippet: .. 260 261 Immunofluorescence staining 262 For mitochondrial internalization in vitro, iPSC-MSCs were pre-labeled with 263 MitoTracker Red CMXRos (Thermo Fisher Scientific, M46752) according to the 264 manufacturer’s instructions, followed by mitochondrial isolation as described above. ..

    Article Title: Therapeutic activity of a hematopoietic stem cell-delivered cell-penetrating frataxin in Friedreich's ataxia models.
    Article Snippet: .. Microscopy analyses and deep SIM imaging Similarly, fibroblasts were analyzed by immunofluorescence microscopy for intracellular frataxin by staining 2 × 104 supernatant treated cells seeded on coverslips with 100nM MitoTracker Red CMXRos (Invitrogen) for 30min at 37◦C, fixing them in 4% paraformaldehyde then permeabilising for 10 min with PBS containing 0.2% Triton X-100. .. The samples were then incubated with a blocking solution of PBS 10% Goat Serum for 30 min hour at 37◦C and stained with anti-frataxin antibody (18A5DB1, Abcam) for 16h at 4◦C.

    Article Title: Assessing cellular metabolic dynamics with NAD(P)H fluorescence polarization imaging
    Article Snippet: .. Cells prepared on glass bottom dishes were retrieved from an incubator, and stained with 1 μM MitoTracker Red CMXRos (Thermo Fisher Scientific Inc., Waltham, MA) in the respective cell line’s growth medium for 10 min. All stained cells were rinsed 3x with fresh phenol red-free, HEPES-buffered DMEM/F12 media to remove excess dye before imaging. .. Cells prepared on glass bottom dishes were retrieved from an incubator, and stained with 1 μM Nile Red (Thermo Fisher Scientific Inc., Waltham, MA) in the respective cell line’s growth medium for 10 min. All stained cells were rinsed 3x with fresh phenol red-free, HEPES-buffered DMEM/F12 media to remove excess dye before imaging.

    Article Title: Metformin promotes lipid droplet-mitochondria coupling and improves insulin secretion in pancreatic β-cells exposed to lipotoxicity.
    Article Snippet: Lipotoxicity that is caused by excess lipid accumulation is a major factor that contributes to gradual impairments of β-cell function and the development of type 2 diabetes.. Metformin has shown protective effects against lipidinduced damage in β-cells, but its specific mechanisms of action within pancreatic islets remain unclear.. The present study comprehensively examined direct effects of metformin on lipid metabolism pathways in INS-1E β-cells that were exposed to lipotoxic stress.

    Mmp Assay:

    Article Title: Reverse Effects of Nicotinamide Mononucleotide Supplementation on Declining Quality of Oocytes With Polycystic Ovary Syndrome
    Article Snippet: .. The probes included dichlorodihydrofluorescein diacetate (Beyotime Biotechnology) for ROS, 500 nM cell‐permeant MitoTracker Red CMXRos (Thermo Fisher Scientific) for mitochondria, 10 μmol/L monochlorobimane (MedChemExpress) for GSH, ATP‐Red 1 (MedChemExpress) for ATP, an enhanced MMP assay kit with JC‐1 (Beyotime Biotechnology) for mitochondrial membrane potential, and Annexin V probes (Beyotime Biotechnology) for apoptosis. ..

    Membrane:

    Article Title: Reverse Effects of Nicotinamide Mononucleotide Supplementation on Declining Quality of Oocytes With Polycystic Ovary Syndrome
    Article Snippet: .. The probes included dichlorodihydrofluorescein diacetate (Beyotime Biotechnology) for ROS, 500 nM cell‐permeant MitoTracker Red CMXRos (Thermo Fisher Scientific) for mitochondria, 10 μmol/L monochlorobimane (MedChemExpress) for GSH, ATP‐Red 1 (MedChemExpress) for ATP, an enhanced MMP assay kit with JC‐1 (Beyotime Biotechnology) for mitochondrial membrane potential, and Annexin V probes (Beyotime Biotechnology) for apoptosis. ..

    Electron Microscopy:

    Article Title: MICU2 controls mitochondrial calcium signaling and migration in neurons during development
    Article Snippet: .. DIV11–15 neurons on 18 or 25-mm coverslips were incubated for 10 min at RT with 200 nM MitoTracker Red CMXROS (Thermo Fisher Scientific, M46752) followed by 3 × 5 min washes with Hanks’ balanced salt solution (HBSS; Gibco, 14025076) and fixation in 4% PFA (Electron Microscopy Sciences, 15714S) prewarmed to 37°C for 15 min at RT. .. Further, the cells were washed 3 × 5 min with PBS, blocked for 1 h at RT in PBS containing 3% BSA, 5% chicken serum, and 0.2% Triton x-100, and stained with primary antibodies (anti-MICU2, Bethyl, A300-BL19212, 1:200) for 45 min at RT.

    Immunofluorescence:

    Article Title: Transplantation of Mitochondria isolated from iPSC-MSCs mitigates doxorubicin-induced cardiomyopathy by inhibiting cardiomyocyte senescence.
    Article Snippet: .. 260 261 Immunofluorescence staining 262 For mitochondrial internalization in vitro, iPSC-MSCs were pre-labeled with 263 MitoTracker Red CMXRos (Thermo Fisher Scientific, M46752) according to the 264 manufacturer’s instructions, followed by mitochondrial isolation as described above. ..

    Article Title: Therapeutic activity of a hematopoietic stem cell-delivered cell-penetrating frataxin in Friedreich's ataxia models.
    Article Snippet: .. Microscopy analyses and deep SIM imaging Similarly, fibroblasts were analyzed by immunofluorescence microscopy for intracellular frataxin by staining 2 × 104 supernatant treated cells seeded on coverslips with 100nM MitoTracker Red CMXRos (Invitrogen) for 30min at 37◦C, fixing them in 4% paraformaldehyde then permeabilising for 10 min with PBS containing 0.2% Triton X-100. .. The samples were then incubated with a blocking solution of PBS 10% Goat Serum for 30 min hour at 37◦C and stained with anti-frataxin antibody (18A5DB1, Abcam) for 16h at 4◦C.

    In Vitro:

    Article Title: Transplantation of Mitochondria isolated from iPSC-MSCs mitigates doxorubicin-induced cardiomyopathy by inhibiting cardiomyocyte senescence.
    Article Snippet: .. 260 261 Immunofluorescence staining 262 For mitochondrial internalization in vitro, iPSC-MSCs were pre-labeled with 263 MitoTracker Red CMXRos (Thermo Fisher Scientific, M46752) according to the 264 manufacturer’s instructions, followed by mitochondrial isolation as described above. ..

    Isolation:

    Article Title: Transplantation of Mitochondria isolated from iPSC-MSCs mitigates doxorubicin-induced cardiomyopathy by inhibiting cardiomyocyte senescence.
    Article Snippet: .. 260 261 Immunofluorescence staining 262 For mitochondrial internalization in vitro, iPSC-MSCs were pre-labeled with 263 MitoTracker Red CMXRos (Thermo Fisher Scientific, M46752) according to the 264 manufacturer’s instructions, followed by mitochondrial isolation as described above. ..

    Concentration Assay:

    Article Title: Modeling the cell biology of PEX11β deficiency during human neurogenesis
    Article Snippet: Cells were washed once with 1X PBS and sealed with Fluoromount-G slide mounting medium (Thermo Fisher Scientific #00-4958-02). .. For MitoTracker experiments, cells were incubated with MitoTracker Red CMXRos (Thermo Fisher Scientific #M46752) at a concentration of 100nm for 30 minutes at 37°C and 5% CO2 prior to fixation. .. Neural rosettes were fixed on day 8 of differentiation with 100% ice-cold methanol (Thermo Fisher Scientific #A454-4) for 10 minutes at -20°C.

    Microscopy:

    Article Title: Therapeutic activity of a hematopoietic stem cell-delivered cell-penetrating frataxin in Friedreich's ataxia models.
    Article Snippet: .. Microscopy analyses and deep SIM imaging Similarly, fibroblasts were analyzed by immunofluorescence microscopy for intracellular frataxin by staining 2 × 104 supernatant treated cells seeded on coverslips with 100nM MitoTracker Red CMXRos (Invitrogen) for 30min at 37◦C, fixing them in 4% paraformaldehyde then permeabilising for 10 min with PBS containing 0.2% Triton X-100. .. The samples were then incubated with a blocking solution of PBS 10% Goat Serum for 30 min hour at 37◦C and stained with anti-frataxin antibody (18A5DB1, Abcam) for 16h at 4◦C.

    Imaging:

    Article Title: Therapeutic activity of a hematopoietic stem cell-delivered cell-penetrating frataxin in Friedreich's ataxia models.
    Article Snippet: .. Microscopy analyses and deep SIM imaging Similarly, fibroblasts were analyzed by immunofluorescence microscopy for intracellular frataxin by staining 2 × 104 supernatant treated cells seeded on coverslips with 100nM MitoTracker Red CMXRos (Invitrogen) for 30min at 37◦C, fixing them in 4% paraformaldehyde then permeabilising for 10 min with PBS containing 0.2% Triton X-100. .. The samples were then incubated with a blocking solution of PBS 10% Goat Serum for 30 min hour at 37◦C and stained with anti-frataxin antibody (18A5DB1, Abcam) for 16h at 4◦C.

    Article Title: Assessing cellular metabolic dynamics with NAD(P)H fluorescence polarization imaging
    Article Snippet: .. Cells prepared on glass bottom dishes were retrieved from an incubator, and stained with 1 μM MitoTracker Red CMXRos (Thermo Fisher Scientific Inc., Waltham, MA) in the respective cell line’s growth medium for 10 min. All stained cells were rinsed 3x with fresh phenol red-free, HEPES-buffered DMEM/F12 media to remove excess dye before imaging. .. Cells prepared on glass bottom dishes were retrieved from an incubator, and stained with 1 μM Nile Red (Thermo Fisher Scientific Inc., Waltham, MA) in the respective cell line’s growth medium for 10 min. All stained cells were rinsed 3x with fresh phenol red-free, HEPES-buffered DMEM/F12 media to remove excess dye before imaging.



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    NRF2 inhibition results in impaired mitochondrial structure and function in Chicken PGCs. GSEA of genes related to mitophagy (A), citrate cycle (B), and oxidative phosphorylation (C) in ML385-PGCs versus Ctrl-PGCs. (D) TEM images showing subcellular structures of ML385-PGCs and Ctrl-PGCs. Yellow arrowheads indicate mitochondria with intact cristae; red arrowheads indicate mitochondria with disrupted cristae and vacuolization. Yellow arrows denote condensed chromatin in the nucleus. Scale bar: 2 μm. Original magnification, × 3. (E) Quantification of mitochondrial number per cell in Ctrl-PGCs and ML385-PGCs based on TEM images ( n = 3 per group). (F) Quantification of the percentage of vacuolated mitochondria in Ctrl-PGCs and ML385-PGCs based on TEM images ( n = 3 per group). (G and H) Assessment of mitochondrial activity using <t>MitoTracker®</t> Red <t>CMXRos</t> staining in ML385-PGCs and Ctrl-PGCs ( n = 3 per group). Scale bar: 50 μm. Statistical significance is indicated by * ( p < 0.05) and *** ( p < 0.001).
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    Subcellular localization of GPP proteins. Confocal laser scanning microscopy images of Arabidopsis cells expressing GFP, GPP1–GFP, or GPP2–GFP. GFP fluorescence, <t>MitoTracker</t> staining, chlorophyll autofluorescence, and merged images are shown. (A) GPP2–GFP or GFP alone. (B) GPP1–GFP or GFP alone.
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    Image Search Results


    NRF2 inhibition results in impaired mitochondrial structure and function in Chicken PGCs. GSEA of genes related to mitophagy (A), citrate cycle (B), and oxidative phosphorylation (C) in ML385-PGCs versus Ctrl-PGCs. (D) TEM images showing subcellular structures of ML385-PGCs and Ctrl-PGCs. Yellow arrowheads indicate mitochondria with intact cristae; red arrowheads indicate mitochondria with disrupted cristae and vacuolization. Yellow arrows denote condensed chromatin in the nucleus. Scale bar: 2 μm. Original magnification, × 3. (E) Quantification of mitochondrial number per cell in Ctrl-PGCs and ML385-PGCs based on TEM images ( n = 3 per group). (F) Quantification of the percentage of vacuolated mitochondria in Ctrl-PGCs and ML385-PGCs based on TEM images ( n = 3 per group). (G and H) Assessment of mitochondrial activity using MitoTracker® Red CMXRos staining in ML385-PGCs and Ctrl-PGCs ( n = 3 per group). Scale bar: 50 μm. Statistical significance is indicated by * ( p < 0.05) and *** ( p < 0.001).

    Journal: Poultry Science

    Article Title: NRF2 deficiency impairs proliferation and survival of chicken primordial germ cells via oxidative stress, mitochondrial dysfunction and apoptosis

    doi: 10.1016/j.psj.2026.106765

    Figure Lengend Snippet: NRF2 inhibition results in impaired mitochondrial structure and function in Chicken PGCs. GSEA of genes related to mitophagy (A), citrate cycle (B), and oxidative phosphorylation (C) in ML385-PGCs versus Ctrl-PGCs. (D) TEM images showing subcellular structures of ML385-PGCs and Ctrl-PGCs. Yellow arrowheads indicate mitochondria with intact cristae; red arrowheads indicate mitochondria with disrupted cristae and vacuolization. Yellow arrows denote condensed chromatin in the nucleus. Scale bar: 2 μm. Original magnification, × 3. (E) Quantification of mitochondrial number per cell in Ctrl-PGCs and ML385-PGCs based on TEM images ( n = 3 per group). (F) Quantification of the percentage of vacuolated mitochondria in Ctrl-PGCs and ML385-PGCs based on TEM images ( n = 3 per group). (G and H) Assessment of mitochondrial activity using MitoTracker® Red CMXRos staining in ML385-PGCs and Ctrl-PGCs ( n = 3 per group). Scale bar: 50 μm. Statistical significance is indicated by * ( p < 0.05) and *** ( p < 0.001).

    Article Snippet: Mitochondrial activity was assessed using the MitoTracker® Red CMXRos Kit (40741ES50, Yeasen, Shanghai, China) according to the manufacturer's instructions.

    Techniques: Inhibition, Phospho-proteomics, Activity Assay, Staining

    Subcellular localization of GPP proteins. Confocal laser scanning microscopy images of Arabidopsis cells expressing GFP, GPP1–GFP, or GPP2–GFP. GFP fluorescence, MitoTracker staining, chlorophyll autofluorescence, and merged images are shown. (A) GPP2–GFP or GFP alone. (B) GPP1–GFP or GFP alone.

    Journal: bioRxiv

    Article Title: AtGPP2 encodes a 3-deoxy- manno- octulosonate-8-phosphatase required for the synthesis of KDO in rhamnogalacturonan II

    doi: 10.64898/2026.05.02.722439

    Figure Lengend Snippet: Subcellular localization of GPP proteins. Confocal laser scanning microscopy images of Arabidopsis cells expressing GFP, GPP1–GFP, or GPP2–GFP. GFP fluorescence, MitoTracker staining, chlorophyll autofluorescence, and merged images are shown. (A) GPP2–GFP or GFP alone. (B) GPP1–GFP or GFP alone.

    Article Snippet: Seedlings were stained with MitoTracker Red CMXRos (Cambrex Bio Science Walkersville) and examined for GFP or MitoTracker fluorescence using a confocal laser scanning microscope (FluoView FV500, Olympus).

    Techniques: Confocal Laser Scanning Microscopy, Expressing, Fluorescence, Staining